UJI AKTIVITAS ANTIMIKROBA BLIS (BACTERIOCIN-LIKE INHIBITORY SUBSTANCE) TERHADAP BAKTERI PENYEBAB PEMBUSUKAN SKIPJACK TUNA (SALMONELLA, S.AUREUS, L.MONOSITOGENIS) MELALUI METODE MIC MBC

Skipjack tuna is one of the fish with the highest production in Indonesia, reaching 537,688 tons in 2022. However, damage and decay that occurs are also increasing over time, spoilage of skipjack tuna is often caused by contamination of pathogenic bacteria such as Salmonella sp., Staphylococcus aureus, and Listeria monocytogenes. This study aims to evaluate the potential of BLIS (Bacteriocin-Like Inhibitory Substance) as a natural antimicrobial compound by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). BLIS is produced from lactic acid bacteria isolates from traditional fish fermentation products. The results showed that BLIS was able to significantly inhibit and kill all three pathogenic bacteria. Salmonella sp. and S. aureus showed the highest sensitivity to BLIS, while L. monocytogenes required a higher concentration to achieve the same effect. Statistical analysis confirmed significant differences in the effectiveness of BLIS against each test bacteria. These findings indicate the potential of BLIS as an alternative natural preservative for fishery products, especially skipjack tuna. The use of BLIS can extend shelf life by inhibiting the growth of spoilage bacteria while reducing dependence on synthetic preservatives.