OPTIMALISASI KULTUR JARINGAN EDELWEIS JAWA (Anaphalis javanica(DC.)Sch.Bip) DENGAN PENAMBAHAN ZPT IAA (Indole Acetic Acid) dan IBA (Indole Butyric Acid)

Edelweiss (Anaphalis javanica (DC.)Sch.Bip), an Indonesian endemic to the alpine zone, is often exploited and thus endangered. The importance of this research is because the availability of Edelweiss in Bromo Tengger Semeru National Park has not met the needs of conservation and commercial. Tissue culture is expected to improve edelweiss propagation using regeneration from other organs without waiting for the seed harvest period. This study was conducted to see the effect of giving Indole Acetic Acid (IAA) and Indole Butyric Acid (IBA) on Murashige & Skoog (MS) media on Edelweiss tissue culture using shoot meristems. This research was conducted at the Biotechnology Laboratory of Muhammadiyah University of Malang, with four Indole Acetic Acid treatments (A1: 0.1 mg/l, A2: 0.3 mg/l, A3: 0.5 mg/l, A4: 1.0 mg/l) and four Indole Butyric Acid treatments (B1: 0.1 mg/l, B2: 0.3 mg/l, B3: 0.5 mg/l, B4: 1.0 mg/l) so that there were 16 treatment combinations for leaf, root, and shoot growth with 60 days of observation. The results of the analysis showed that there were several treatment combinations that produced a live percentage of 100%, the highest combination of shoot growth was IAA 0.5 mg/l and IBA 0.3 mg/l, the highest combination of root growth IAA 0.3 mg/l and IBA 0.1 mg/l, the highest combination of leaf growth IAA 0.3 mg/l and IBA 0.5 mg/l. The results showed that the optimal concentration for edelweiss growth is a combination of IAA 0.3 mg/l and IBA 0.5 mg/l, the combination increases edelweiss propagation so that it can meet conservation and commercial needs.