PERTUMBUHAN MIKROPLANTLET ANGGREK Laeliocattleya Prism Pallete PADA MEDIA HYDROGEL – MOSS DENGAN PEMBERIAN KONSENTRASI PUPUK MAJEMUK NPK

This research aims to examine the effect of the combination of growing media and the application of compound fertilizer on the growth of Laeliocattleya Prism Pallete microplantlets in ex-vitro subculture. The purpose of this study is to provide information about the optimal concentration of compound fertilizer and growing media composition for the growth of Laeliocattleya orchid microplantlets. It is hypothesized that there is an interaction between the combination of hydrogel and moss growing media compositions and the concentration of compound fertilizer on the growth of Laeliocattleya Prism Pallete microplantlets in ex-vitro subculture. The research was conducted in Junrejo Village, Junrejo District, Batu City, at an altitude of approximately 700 meters above sea level, from November 2021 to March 2022. The equipment used in the research included tweezers, color scales, documentation tools, writing tools, rulers, measuring cups, sprayers, buckets, and scales. The materials used in this research were Laeliocattleya Prism Pallete hybrid orchid microplantlets, sphagnum moss growing media, hydrogel, root stimulant plant growth regulator, compound fertilizer (NPK 17:11:10), and fungicide. This research was carried out using a Randomized Complete Block Design (RCBD) arranged in a factorial design with 2 factors and 3 replications. The first factor was the combination of growing media (M) consisting of 7 types, namely: M1: 20% hydrogel and 80% moss, M2: 30% hydrogel and 70% moss, M3: 40% hydrogel and 60% moss, M4: 50% hydrogel and 50% moss, M5: 60% hydrogel and 40% moss, M6: 70% hydrogel and 30% moss, M7: 80% hydrogel and 20% moss. The second factor was the concentration of compound fertilizer NPK (P) consisting of 4 levels, namely P1: concentration of NPK fertilizer 0.5 g/L, P2: concentration of NPK fertilizer 1 g/L, P3: concentration of NPK fertilizer 1.5 g/L, P4: concentration of NPK fertilizer 2 g/L. Based on the research results, it can be concluded that there is an interaction between the combination of hydrogel and sphagnum moss growing media compositions and the concentration of compound fertilizer NPK on the growth of microplantlets in ex-vitro subculture with variabels such as number of leaves, leaf width, and length of new roots. The optimal treatment combinations for the number of new leaves variabel are 70% hydrogel and 30% moss composition plus 2 g/L fertilizer concentration, 70% hydrogel and 30% moss composition plus 1.5 g/L fertilizer concentration. For the variabel of root length, the optimal treatment is 70% hydrogel and 30% moss composition plus 0.5 g/L fertilizer concentration. The growing media composition that can assist in the growth of microplantlets for the number of leaves variabel is 50% hydrogel and 50% moss composition, leaf length in 70% hydrogel and 30% moss composition, leaf width in 70% hydrogel and 30% moss composition. The application of compound fertilizer has a significant effect on the growth of microplantlets in ex-vitro subculture in terms of the number of new roots variabel. The optimal concentration of compound fertilizer is 2 g/L.