Diversity of Phaseolus lunatus L. in East Java, Indonesia based on PCR-RAPD Technique

Phaseolus lunatus L. is one of the legume plants found in some parts of Indonesia and has potential as alternative food rich
in protein. This current research aimed at analysing genetic accessions of P. lunatus distributed in some areas in East Java,
Indonesia, based on the RAPD (Random Amplified Polymorphic Deoxyribonucleic acid) marker. 15 accessions originated
from four locations were analysed. Ten primers were used and produced 68 bands out of 67 were polymorphic. The percent
polymorphism was 96% to 100%. Ten unique bands were detected in eight accessions (Prb1, Prb2, Prb5, Mdr12, Mdr16,
Mdr19, Mdr4, and Mdr6). Using the Neighbor-Joining method, a phylogenetic tree was yielded by a similarity coefficient of
64% to 100%. On the genetic similarity coefficient (GSC) of 0.6, there were two clusters: the first and second major clusters
(Cluster A and B). The former contained the accessions 7, 8, 13, and 14, while the latter comprised 2, 4, 16, 18, Prb1, Prb2,
Prb3, Prb4, and Prb5. In conclusion, based on phylogenetic trees formed, P. lunatus from the same region cluster in the same
cluster.