PENGARUH DOSIS STIGMASTEROL DAUN BELUNTAS (Pluchea indica) TERHADAP TOKSISITAS AKUT TIKUS PUTIH JANTAN (Rattus norvegicus) SEBAGAI SUMBER BELAJAR

Beluntas plants are often used as traditional medicine. Stigmasterol is an active compound in the sterois group that has potential as an antifertility agent. This study aims to determine the effect of stigmasterol from beluntas leaves on the behavior of male white
rats after treatment with various doses. The study was conducted at the Biomedical Laboratory of Muhammadiyah University Muhammadiyah Malang over a period of 28 days. The study design employed a experimental design using a RAL experimental design,
consisting of four treatment groups and six replicates. The stigmasterol doses for each treatment were (0.125 mg/kg body weight), (0.25 mg/kg body weight), and (0.5 mg/kg body weight). Behavioral observations were conducted on parameters including body posture, motor activity, ataxia, righting reflex, kasa test, and analgesia over 24 hours. The results demonstrated that stigmasterol did not cause significant acute toxic effects in male white rats based on the observed parameters. This study has the potential to serve as a learning resource for clinical pharmacy and community pharmacy courses in Grade XI of Pharmacy Vocational High School on the topic of standardized herbal medicines.