Liquid culture for efficient in vitro propagation of potato (Solanum tuberosum L.) using bioreactor system

The bioreactor is one method of in vitro culture for plant propagation. Some plants that have used this method in propagation are date palms, ornamental plants, and medicinal plants, but potato seed production has not yet been carried out. In vitro culture activities usually use solid or semi-solid media, namely culture media with the addition of agar. In a bioreactor system, the media used does not use agar so it is called liquid media. Propagation methods using tissue culture generally use a non-bioreactor culture system which causes production prices to increase. Therefore, it is interesting to use a bioreactor system in potato seed production. The research aimed to compare the best combination of bioreactor system treatment and non-bioreactor culture system with the addition of IBA for the growth and development of Amudra potato seed plantlets. The experimental design used a Complete Randomized Design (CRD) with 6 treatments, and 4 repetitions. The treatments in this study were non-bioreactor without IBA (control), non-bioreactor + IBA 1 mg L-1, non-bioreactor + IBA 2 mg L-1, bioreactor without IBA (control), bioreactor + IBA 1 mg L-1, and bioreactor + IBA 2 mg L-1. Bioreactor treatment (control) is better than other treatments. This can be seen from the number of shoots 7.30, stem diameter of 2.83 mm3, leaf area of 1.42 cm2, number of leaves of 9.60, and total chlorophyll content of 0.32 mg L-1. The results indicate that bioreactor culture with a liquid medium could be used for mass micropropagation of S. tuberosum L.