Molecular Characteristic of Fusarium oxysporum from Different Altitudes in East Java, Indonesia

Tomato (Solanum lycopersicum L.) is one of the most economically important vegetable crops in Indonesia. Tomato diseases caused by fungi are transmitted by seed or transplants. Fusarium wilt disease is a cosmopolitan species caused by Fusarium oxysporum Schlecht. Emend. Snyder & Hansen. Among this special attention of disease caused by F. oxysporum has been given to stem and root rotting. Six selected Fusarium samples from previous research were prepared using a single spore method and cultured in the PDB medium, The Research carried out in the Agrotechnology Laboratory of the University of Muhammadiyah Malang. DNA extraction and PCR used ITS 1 and ITS 4, electrophoresis, and data analysis was achieved at the Genetic and Molecular Laboratory of the Biology Department of the Faculty of Science and Technology, Maulana Malik Ibrahim Malang Islamic State University. Isolate code 3313439 originating from Karangploso soil (515 m a.s.l) and code 3313428 derived from the soil of Blitar (156 m a.s.l.) showed species similarity to F. oxysporum f. sp lycopersici strain CBS249.52. Then for sample 3313426, the roots of Pujon have similarities with the strain of F. oxysporum S58. Besides, samples of 3313 422 Blitar roots, 3313 424 Karangploso roots, and 3313 432 Pujon soils (956 m a.s.l.) showed proximity to species F. oxysporum f.sp. pisi HG423346. The samples were in one clade with the nucleotide base sequences of two other F. oxysporum species recorded in the NCBI Genbank database. Differences in species will likely affect the pathogenicity, growth rate, spore production, and disease control management.