Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018)

The purpose of this research was to investigate the
ability of rice endophytic bacteria to synthesize extracellular
metabolites before and after gfp (Green Fluorescent Protein)
gene transformation. These bacteria were grown in liquid media
M63+2mM Tryptophan for 3 days and the supernatants were
extracted with absolute methanol 1:1 (v/v). The extractants were
then
analysed
with
Gas
Chromatography-Mass
Spectrophotometry (GC-MS). The GC-MS result showed that all
isolates that were tested be it the control (without
transformation) and the mutants (transformed) were able to
synthesize growth hormones with different composition
patterns, namely Indole 3 Butryric Acid (IBA), Indole Acetic
Acid (IAA), Kinentin, Cis Zeatin, Zeatin, Benzylaminopurine,
Giberellic Acid (GA3, GA1) as well as Zeatin Riboside.
Furthermore, the isolates also synthesised osmoprotectants,
namely Glycine, Proline, Arabinose, Thiamine, and Trehalose
with different composition patterns as well.