Identifikasi Bakteri Metanotrof pada Hutan Mangrove dan Hutan Produksi di Desa Tambakrejo Kecamatan Sumbermanjing Wetan Kabupaten Malang

Greenhouse Gas (GHG) balance has
an impact on the earth's environment because it can reduce global warming and extreme climate change.
CH4 is a greenhouse gas that can cause an 80% increase in global warming potential over the last 20 years.
Methanotrophic bacteria have monooxygenase enzymes which are used in the methane oxidation process.
Bacteria that are active in aerobic conditions have an important role in the methane gas cycle and are a
solution for reducing methane emissions in the air. The purpose of this study was to identify the isolate
genus of methanotrophic bacteria and to analyze the macroscopic conditions and biochemical properties of
methanotrophic bacteria in mangrove and production forest areas. The method of this research was to isolate
methanotrophic bacteria from the five soil samples in the mangrove forest and production forest areas.
Isolation of methanotrophic bacteria using NMS (Nitrate Mineral Salts) selective media. Growing bacterial
colonies were observed macroscopically (color, shape, size, elevation, topography and optical properties),
biochemical properties (gram test and catalase test), as well as data analysis using qualitative and
quantitative descriptive methods. There was no fundamental difference in examining the macroscopic
properties of the two areas (mangrove forest and production forest). There are 3 genera of methanotrophic
bacteria in the mangrove forest area, namely Methylophaga, Methylobacterium, and Methylococcus.
Meanwhile, in production forests there are 2 genera, namely Methylobacterium and Methylovorus.