Optimization of indigenous rhizobacteria in tomato and potato rhizosphere as a source of cytokinins to reduce drought stress

The use of organic materials is currently a concern in developing plant commodities. Important organic materials such as fertilizers, pesticides, and phytohormones. The phytohormone cytokinin is one of the important hormones for plants because cytokinin can help growth and make plants tolerant to abiotic stress. One that can produce cytokinin is rhizobacteria. This study explores the potential of rhizobacteria as producers of cytokinin phytohormone against plant abiotic stress. Rhizobacteria from tomato and potato plants were isolated using standard m63 media and tested for metabolomics using GC-MS. The results showed that these rhizobacteria can produce various cytokinin derivative metabolites such as Trans Zeatin, cis Zeatin, and Kinetin. These metabolites function to increase plant tolerance to abiotic stress. This study uses a bioinformatic approach to GC-MS results to map the characteristics of metabolites that are potential for plants to deal with abiotic stress so that they can become a development strategy to increase productivity under abiotic stress conditions. The results showed that the three isolated bacteria produced higher concentration of kinetin 7N glucosinolate form compared to other cytokinin derivatives. The concentrations were 2,79%, 2,08% and 2,14% in isolate IRT1 10-3, IRT3 10-2 and IRT3 10-4, respectively.