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THE EFFECT OF CONCENTRATION OF POLYETHILENE GLYCOL (PEG) ON PROLIN content of POTATO PLANTS (Solanum tuberosum L.) IN VITRO

Kurniawati, Rina (2019) THE EFFECT OF CONCENTRATION OF POLYETHILENE GLYCOL (PEG) ON PROLIN content of POTATO PLANTS (Solanum tuberosum L.) IN VITRO. Bachelors Degree (S1) thesis, University of Muhammadiyah Malang.

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Abstract

Potatoes (Solanum tuberosum L.) are plants that have priority development. Potatoes are used as a carbohydrate source, highly nutritious, especially vitamins and minerals and have potential in food diversification. Market demand tends to increase, while potato productivity is still low. One of the low causes is global warming which causes erratic climate change and declining availability of ground water. This makes planting land not ideal for potato plant growth, so it is necessary to select potato plants that are tolerant to drought stress. The administration of PEG in explants aims to create drought stress conditions in the environment around explants. This study aims to obtain information on the effect of PEG concentration on potato plant growth and obtain information on the pattern of proline synthesis in various PEG treatments in potato plants (Solanum tuberosum L.) in vitro. This research was carried out at the tissue culture laboratory, the Biotechnology Development Center of the University of Muhammadiyah Malang starting in January 2019 until May 2019. The design of the study was a simple RAL. The treatment used in this study was P0 = without PEG, P1 = PEG concentration of 13%, P2 = PEG concentration of 14%, P3 = PEG concentration of 15%, P4 = PEG concentration of 16%, and P5 = concentration of 17%. The variables of this study LD50 test stage are the percentage of explants live, at the selection stage are explants height, number of explant leaves, number of explant leaves, number of secondary roots, shoot time, percentage of live explants, percentage of explants die, proline content, stomatal appearance (length and stomata appearance). width), appearance of vessel tissue. The results of this study indicate that Lethal Dose 50 (LD50) PEG concentration in potato plants is 15%. The right concentration for selecting potato explants is 13%, 14%, and 15%. The treatment of increasing PEG concentrations can reduce the growth of potato explants to the variable number of leaves, plant height, number of secondary roots. But it increases the time when buds appear and proline content.

Item Type: Thesis (Bachelors Degree (S1))
Student ID: 201510200311115
Thesis Advisors: Maftuchah (NIP 196803121992121002), Ali Ikhwan (NIP 196410201991011001)
Keywords: Potatoes (Solanum tuberosum L.), PEG concentration, proline
Subjects: S Agriculture > SB Plant culture
Divisions: Faculty of Agriculture and Animal Husbandry > Department of Agrotechnology (54211)
Depositing User: 201510200311115
Date Deposited: 24 Aug 2019 02:15
Last Modified: 24 Aug 2019 02:15
URI : http://eprints.umm.ac.id/id/eprint/51846

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