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UJI AKTIVITAS ANTIBAKTERI FRAKSI ETIL ASETAT UMBI Eleutherine palmifolia (L.) TERHADAP Staphylococcus aureus DENGAN METODE DIFUSI CAKRAM

Anwar, Zakiyahtul Kiftiyah (2019) UJI AKTIVITAS ANTIBAKTERI FRAKSI ETIL ASETAT UMBI Eleutherine palmifolia (L.) TERHADAP Staphylococcus aureus DENGAN METODE DIFUSI CAKRAM. Bachelors Degree (S1) thesis, University of Muhammadiyah Malang.

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Abstract

Background: One of the infections that caused by Staphylococcus bacteria is pneumonia bacteriologic. Resistance with antibiotics oftenly happens, therefore need an alternative traditional treatment that is Eleutherine palmifolia. Objective: the aim of this research is to find out the classes of secondary metabolites that found on ethyl acetate fraction at Eleutherine palmifolia tubers and obtain data of inhibition zone ethyl acetate fraction Eleutherine palmifolia to Staphylococcus aureus growth. Method: to find out an antibacterial activity test using disc diffusion methods E. palmifolia tuber ethyl acetate fraction with maceration level methods, then secondary metabolites identified with KLT methods and doing a test of bacterial activity with disc diffusion methods. The concentration of solvent test is 20 mg/ml, 40 mg/ml, and 60 mg/ml. Negative control is DMSO 1% and aquadest sterilization, while positive control is cloramphenicol 30 µg/disc. Bacteria suspension swiped on solidify jelly as the media. Result and Conclusion: the result of secondary metabolites identification, ethyl acetate fraction of Eleutherine palmifolia contains alkaloid, flavonoid, terpenoid, polifenol, and antrakinon. The result of antibacterial activity testing of ethyl acetate fraction on Eleutherine palmifolia tubers to Staphylococcus aureus with 20 mg/ml(0,6 mg/disk) concentration has inhibition zone 24,25 mm, 40 mg/ml(1,2 mg/disk) concentration has inhibition zone 27,46 mm, and 60 mg/ml(1,8mg/disk) concentration has inhibition zone 28,16 mm. Eventually, it can be concluded that ethyl acetate fraction of Eleutherine palmifolia tubers has an activity as antibacterial.

Item Type: Thesis (Bachelors Degree (S1))
Student ID: 201510410311080
Thesis Advisors: Siti Rofida (0728087904), Ahmad Shobrun Jamil (0721018502)
Keywords: antibacterial, ethyl acetate fraction, Eleutherine palmifolia tubers, Staphylococcus aureus, Disc diffusion
Subjects: L Education > L Education (General)
R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Health Science > Department of Pharmacy (48201)
Depositing User: 201510410311080
Date Deposited: 15 Aug 2019 03:16
Last Modified: 15 Aug 2019 03:16
URI : http://eprints.umm.ac.id/id/eprint/48945

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