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Endoglucanase, exo-glucanase and â-glucosidase activity thecombination of crude enzyme from Trichoderma reesei and Aspergillus niger at different temperatures and pH

Argo, Bambang Dwi and Wardani, Agustin Krisna and Zubaidah, Elok and Winarsih, Sri (2014) Endoglucanase, exo-glucanase and â-glucosidase activity thecombination of crude enzyme from Trichoderma reesei and Aspergillus niger at different temperatures and pH. Research and Review in BioScience, 9 (2). pp. 45-48. ISSN 0974 - 7532

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Argo Wardani Zubaidah Winarsih - Cellulase enzyme FPAse CMCAse beta-glucosidase Trichoderma reesei Aspergillus niger.pdf

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Abstract

The purpose of this study was to compare the activity of the crude cellulase enzyme produced by Trichoderma reesei and Aspergillus niger and the various combination of two crude enzyme with a ratio of Trichoderma reesei and Aspergillus niger (0/1, 1/0; 1/1; 1/2, 2 / 1; 1/3; 3/1 v / v) at different pH and temperature. The results showed that the highest exo-glucanase activity (FP ase), endo-glucanase activity (CMC ase) and â-glucosidase activity of combined enzyme of Trichoderma reesei : Aspergillus niger in sequence (2 : 1) at pH 5 is 1.002 IU / ml; (1 : 3) at pH 5 is 2.90 IU/ml and (1 : 3) at pH 4.5, generated 0.316 IU/ml and three of those activity reached the highest level at temperatures of 50

Item Type: Article
Keywords: Cellulase enzyme FPAse; CMCAse; beta-glucosidase; Trichoderma reesei; Aspergillus niger.
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Agriculture and Animal Husbandry > Department of Food Technology (41203)
Depositing User: Atik Yuliawati
Date Deposited: 03 Jan 2019 04:37
Last Modified: 03 Jan 2019 04:43
URI : http://eprints.umm.ac.id/id/eprint/42821

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