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UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA KULIT BUAH Citrus reticulata TERHADAP BAKTERI Escherichia coli DENGAN METODE DIFUSI CAKRAM

Ibrahim, Rahmat (2018) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA KULIT BUAH Citrus reticulata TERHADAP BAKTERI Escherichia coli DENGAN METODE DIFUSI CAKRAM. Bachelors Degree (S1) thesis, University of Muhammadiyah Malang.

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Abstract

Background: Citrus reticulata fruit peel is proved to inhibit the growth of Escherichia coli bacteria, the compounds responsible for antibacterial activity are favonoid, alkaloids, polyphenols, and anthrides. Objective: The objective of this study was to look at the inhibitory zone of the Citrus reticulata fruit peel fraction on the growth of Escherichia coli bacteria with various concentrations. Methods: Antibacterial activity test was performed using a disk diffusion method. The n-hexane fraction used in this study was obtained from previous studies.Furthermore, the antibacterial activity test was performed. the concentration of fraction of n-hexane used 5%; 10%; 50%. The disc containing test solution, negative control and positive control were placed on the plate containing Escherichia coli and MHA media, then incubated at 37C for 18 hours and the clear zone formed was measured in diameter. Results and Conclusions: The results of secondary metaabolid identification in previous studies showed pharkation of n-hexane Citrus reticulata fruit buds containing flavonoids, alkaloids, polyphenols and anthrons. Results of antibacterial activity of inhibitory zone diameter with concentration of 4 mg / disk was 9.22 mm, 8 mg / disk 11.03 mm, and 20 mg / disk obtained 10.98 mm.. These results show the fracture of n-hexane skin of Citrus reticulata can inhibit the growth of Escherichia coli bacteria seen from the clear zone around the blank disk.

Item Type: Thesis (Bachelors Degree (S1))
Student ID: 201310410311024
Keywords: n-hexane fraction, Citrus reticulata fruit peel, Antibacterial, Disc diffusion, Escherichia coli.
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculty of Health Science > Department of Pharmacy (48201)
Depositing User: Nurhadin Nurhadin
Date Deposited: 26 Dec 2018 04:42
Last Modified: 26 Dec 2018 04:42
URI : http://eprints.umm.ac.id/id/eprint/42581

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