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Optimasi Konsentrasi Higromisin B sebagai Agen Seleksi Aksesi Jarak Pagar (Jatropha curcasL.) Transgenik pada Tingkat Kecambah

Permatasari, Septiya Dwi (2018) Optimasi Konsentrasi Higromisin B sebagai Agen Seleksi Aksesi Jarak Pagar (Jatropha curcasL.) Transgenik pada Tingkat Kecambah. Bachelors Degree (S1) thesis, University of Muhammadiyah Malang.

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Abstract

Jatropha (Jatropha curcas L.) is a plant that receives a lot of attention because it is able to produce vegetable oils that can be used as alternative fuel. In order to improve the plant traits of jatropha to fulfill the people needs then done through pollen tube transformation. Genetics transformation of plant has any terms that must be met on teir process, one of the terms that must be met it was selection. Selection that used in this study is negative selestion that used hygromycin B as selection agent. Higromycin B is used to select the plant that have a hpt gene. The aim of this study is to determine the 100% lethal concentration of hygromycin B in phase I and to investigate resistance of jatropha transgenic sprout at the 100% lethal concentration in phase II. The material that used in this study was jatropha wild type sprouts that soaked in hygromycin B (0, 35, 40, 45, 50, 55, 60, 65, 70 μg mL-1) with 3 repetition and transgenic sprouts that soaked in hygromycin B (50% and 100% lethal dose in phase I) with 9 repetition. Both of the study used Randomized Complete Block Design (RCBD) then analized using HSD 5%. Hygromycin B affected browningg occur in radicle of wild type sprouts and transgenic sprouts. Soaking in hygromycin B affected to the percentage of brown sprouts, percentage of sprouts appear cotyledons, number of normal sprouts, and number of abnormal sprouts in the jatropha wild type sprouts, but not significantly different in the number of live sprouts from 1st-7th DAP. In phase I is known the 50 and 100% lethal is 65 and 70 μg mL-1 concentration of hygromycin B on jatropha wild type sprouts. Then 50% and 100% lethal concentration tested on jatropha transgenic sprouts, from the result of testing show that there is 1 sample that alive in hygromycin B concentration 70 μg mL-1 in repetition 8 sample 1 that suspected have a hptII gene.

Item Type: Thesis (Bachelors Degree (S1))
Student ID: 201310200311110
Keywords: Hygromycin B, sprouts, transgenik, wild type
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Agriculture and Animal Husbandry > Department of Agrotechnology (54211)
Depositing User: Agung Gallant Setiabudi
Date Deposited: 07 Dec 2018 01:58
Last Modified: 07 Dec 2018 01:58
URI : http://eprints.umm.ac.id/id/eprint/41594

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