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EFEK ISOFLAVON FITOESTROGEN DARI EKSTRAK Pueraria lobata TERHADAP MEMORI DAN AKTIVITAS KOLINERGIK DI HIPPOKAMPUS CA1 PADA TIKUS HIPOESTROGEN

Safithri, Fathiyah and Ali, Mulyohadi and Hidajat, Ahmad and Suharto, Setyawati (2010) EFEK ISOFLAVON FITOESTROGEN DARI EKSTRAK Pueraria lobata TERHADAP MEMORI DAN AKTIVITAS KOLINERGIK DI HIPPOKAMPUS CA1 PADA TIKUS HIPOESTROGEN. Saintika Medika, 6 (13). pp. 28-41. ISSN 0216-759x

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Abstract

Data from retrospective and case-controle showed that estrogen replacement therapy (ERT) could prevent delayed against Alzheimer Disease (AD). The using of ERT is still controversial because of their long-term side effect. Isoflavon as a part of phytoestrogen has potential effect as an alternative substitute for ERT. The aim of this study is to examine the effect of phytoestrogen from Pueraria lobata extract in increasing memory’s function and whether it occur by the increased of cholinergic activity in CA1 hippocampus. Exploration study performed by three step. First, determined genistein and daidzein concentration in Pueraria lobata extract, as basic of dose determining.Second, oophorectomy performed to made a hypoestrogenic rat’s model; and third, determined method memory’s function in rats. This study used twenty five female Rattus novergicus strain Wistar, 10 -11 weeks of age, five rats were normal rats (N) as first group and twenty rats were hypoestrogenic rats for 2nd, 3rd, 4th , and 5th groups. All of the hypoestrogenic rat were devided into 4 groups, each 5 rats. Hypoestrogenic rats group without Isoflavon Genistein Daidzein supplement (OVX), hypoestrogenic rats group with Isoflavon Genistein Daidzein supplement (OVX+IGD) dosage of 15, 30, and 60 mg/kgweight/day each. The IGD was given for 21 days. The Morris Water. Morris (MWM) test was performed in 17th , 18th, 19th, 20th, and 21st day. On 22nd day rats were killed. Frozen section of the brain was performed immediately. Cholinergic activities on CA1 hippocampus were examined with AChE staining. A significant difference was found on memory’s function and cholinergic activities on CA1 hippocampus between OVX and OVX+IGD groups at all doses (p value = 0,000of each). No significant difference on IGD effect toward brain weight (p=0,936) and pyramidal cell number on CA1 hippocampus(p=0,961). It was suggest that increased memory’s function on OVX+IGD groups were caused by the increased of cholinergic activities on CA1 hippocampus.

Item Type: Article
Keywords: Isoflavon, Fitoestrogen, Genistein, Daidzein, Pueraria lobata, Memori, Kolinergik.
Subjects: R Medicine > RC Internal medicine
R Medicine > RM Therapeutics. Pharmacology
Divisions: Faculty of Medicine > Department of Medicine (11201)
Depositing User: Fathiyah Safithri
Date Deposited: 01 Dec 2018 02:59
Last Modified: 01 Dec 2018 02:59
URI : http://eprints.umm.ac.id/id/eprint/41223

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