UMM Institutional Repository

ANALISIS KERAGAMAN GENETIK DELAPAN AKSESI SORGUM (Sorghum bicolor (L.) Moench) DAERAH JAWA TIMUR MENGGUNAKAN PENANDA RAPD

Abdillah, Dian (2018) ANALISIS KERAGAMAN GENETIK DELAPAN AKSESI SORGUM (Sorghum bicolor (L.) Moench) DAERAH JAWA TIMUR MENGGUNAKAN PENANDA RAPD. Bachelors Degree (S1) thesis, University of Muhammadiyah Malang.

[img]
Preview
Text
Pendahuluan.pdf

Download (1MB) | Preview
[img]
Preview
Text
BAB I.pdf

Download (201kB) | Preview
[img]
Preview
Text
BAB II.pdf

Download (635kB) | Preview
[img]
Preview
Text
BAB III.pdf

Download (471kB) | Preview
[img] Text
BAB IV.pdf
Restricted to Repository staff only

Download (1MB)
[img] Text
BAB V.pdf
Restricted to Repository staff only

Download (38kB)
[img] Text
Lampiran.pdf
Restricted to Repository staff only

Download (1MB)

Abstract

Sorghum is the fifth most important cereal crops globally after wheat, rice, maize and barley. It is potentially developed in Indonesia as a diversification of crops and raw material of biofuels. Sorghum have well adaptation in its habitat. Development of local sorghum in Indonesia is low. Genetic diversity observations of local sorgum in East Java have never been applied. In order to develop superior cultivar, identification of genetic diversity for gaining information of sorgum germplasm is necessary to be implemented as the first step of plant breeding. The present study aims to analyze the genetic diversity of eight sorghum accessions in East Java using RAPD markers. This study was carried out at Plant Molecular Laboratory, Biotechnology Development Center/Pusat Pengembangan Bioteknologi (PUSBANG BIOTEK), University of Muhammadiyah Malang. Eight sorghum accessions was obtained from Pasuruan, Sampang, Jombang, Lamongan, Tuban, and Tulungagung. Genomic DNA was extracted from young leaf using CTAB method. The extracted DNA was qualitatively and quantitatively analyzed and was amplified by PCR using 5 RAPD primers namely OPA 02, OPA 10, OPA 13, OPA 19, and OPA 20. The efectiveness of primers was seen through the level of polymorphism. Profile of generated bands was converted as binary data for analyzing the genetic diversity using cluster analysis on NTSYSpc 2.02 program. A dendogram was constructed based on the binary data of five primers using UPGMA method with Dice similarity coefficient. The results of present study showed that the purity levels of extracted DNA were between 1,886-2,625 and between 0,625-1,275 µg/µl for DNA concentrations. The polymorphism level reachs the number of 90%. Amplification of DNA using five RAPD primers revealed that there were high genetic diversity in eight sorgum accessions. The UPGMA dendogram devided the accessions into 2 major groups (group I and II) at 0,57 Dice similarity level and each groups contained 4 accessions.

Item Type: Thesis (Bachelors Degree (S1))
Student ID: 201310200311070
Keywords: DNA Bands, Primer, Similarity, UPGMA
Subjects: Q Science > QH Natural history > QH426 Genetics
S Agriculture > S Agriculture (General)
Divisions: Faculty of Agriculture and Animal Husbandry > Department of Agrotechnology (54211)
Depositing User: Agung Gallant Setiabudi
Date Deposited: 07 Nov 2018 09:03
Last Modified: 07 Nov 2018 09:03
URI : http://eprints.umm.ac.id/id/eprint/39559

Actions (login required)

View Item View Item
UMM Official

© 2008 UMM Library. All Rights Reserved.
Jl. Raya Tlogomas No 246 Malang East Java Indonesia - Phone +62341464318 ext. 150, 151 - Fax +62341464101
E-Mail : infopus[at]umm.ac.id - Website : http://lib.umm.ac.id - Online Catalog : http://laser.umm.ac.id - Repository : http://eprints.umm.ac.id

Web Analytics

UMM Institutional Repository is powered by :
EPrints Logo