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UJI PLASMID Bradyrhizobium japonicum TERHADAP LIMA JENIS PESTISIDA NABATI

Puspitasari, Rahayu (2018) UJI PLASMID Bradyrhizobium japonicum TERHADAP LIMA JENIS PESTISIDA NABATI. Bachelors Degree (S1) thesis, University of Muhammadiyah Malang.

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1. Cover + pernyataan.pdf

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2. BAB I.pdf

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3. BAB II.pdf

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Abstract

Bradyrhizobium japonicum is a rod-shaped gram-negative bacteria that lives on the ground and is symbiotic with soybean plants. These bacteria infect plant roots so that nodules form which help the plant to produce its own nitrogen. Plasmids are circular DNA molecules and are able to replicate freely in the bacterial cytoplasm. Plasmids also carry resistance genes which are useful as selectors of bacterial cells containing recombinant plasmids. But in the process of cultivation of plants, pesticides are often carried out, one of them is biological pesticides so that it is feared that it will kill bacteria and damage the plasmid Bradyrhizobium japonicum. With the damage of the Bradyrhizobium japonicum plasmid, the nodulation process will be inhibited, and the plant cannot meet its need for nitrogen. Based on this, it is necessary to test plasmid Bradyrhizobium japonicum on 5 types of biological pesticides that are often used by farmers, namely mimba leaf pesticides (H1), chopped tobacco (H2), papaya (H3), soursop (H4), and garlic tubers (H5). Isolates were cultured on peptone medium which had been given pesticide treatment for 5 days in a shaker with a rotating speed of 125 rpm. After 5 days, the isolate was harvested and isolated using a centrifuge at a speed of 13,000 rpm. After obtaining DNA pellets, plasmid DNA was then run using agarose gel electrophoresis on a 100 volt voltage for 50 minutes. The running results are then photographed using a gel document so that the DNA band is formed or not. After taking photos with gel doc, it was found that there were DNA bands on all Bradyrhizobium japonicum isolates with different thicknesses. This means that Bradyrhizobium japonicum isolates are resistant to these 5 types of biological pesticides, although the amount varies from high to low. In addition, based on the results of the photo, the size of the plasmid Bradyrhizobium japonicum tested was not much different from the control which meant that the application of biological pesticides to plants would not damage the plasmid Bradyrhizobium japonicum and plants could still meet their nitrogen needs

Item Type: Thesis (Bachelors Degree (S1))
Student ID: 201010200311052
Keywords: Bradyrhizobium japonicum, plasmids, bacteria
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Agriculture and Animal Husbandry > Department of Agrotechnology (54211)
Depositing User: Agung Gallant Setiabudi
Date Deposited: 23 Oct 2018 07:51
Last Modified: 23 Oct 2018 07:51
URI : http://eprints.umm.ac.id/id/eprint/38292

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