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ANALISIS SAKARIN SEBAGAI PEMANIS SINTETIS DALAM MINUMAN DENGAN METODE KLT-DENSITOMETRI (Penelitian dilakukan di Sekolah Dasar Negeri Kecamatan Klojen, Malang )

KUSUMAWATI,, ETIKA WAHYUNING (2013) ANALISIS SAKARIN SEBAGAI PEMANIS SINTETIS DALAM MINUMAN DENGAN METODE KLT-DENSITOMETRI (Penelitian dilakukan di Sekolah Dasar Negeri Kecamatan Klojen, Malang ). Other thesis, University of Muhammadiyah Malang.

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Abstract

The analysis of saccharine in beverages sold in elementary schools Klojen Malang district has been done. This study identified the presence of saccharine and determined the level concentration by thin layer chromatography- densitometry method. The TLC method used silica gel 60 F254 plate as the stationary phase, and a mobile phase composed of chloroform: glacial acetic acid (70: 30). Out of the seven samples, it was found that four samples were positively contained saccharine. The spots had similar Rf values 0,66 to 0,67, and similar spectra pattern, λmax 275nm and their MF (Match Factor) were more than 900. Relationship between the concentration and the area of saccharine spots was evaluated by linear regression with r> 0.9682.Average levels of the six replication were S3: 77.37 mg / L, S4: 76.00 mg / L, S5: 73.06 mg / L, and S7: 99.62 mg / L. According to Minister of Health of the Republic of Indonesia regulation No.722/Menkes/Per/IX/1988 the maxima allowable concentration of artificial sweeteners saccharine in beverage that was equal to 300 mg / L. So it could be concluded those levels of saccharine still below the maximum level. Keywords: beverages, saccharine, TLC-densitometry

Item Type: Thesis (Other)
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Health Science > Department of Pharmacy (48201)
Depositing User: Mr Moch. sholeh
Date Deposited: 26 Apr 2016 03:06
Last Modified: 26 Apr 2016 03:06
URI : http://eprints.umm.ac.id/id/eprint/28157

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